星云棋牌官网下载

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                      液相芯片技术在水产品检疫性病毒检测技术中的运用

                      时间:2019-03-21 来源:畜牧与兽医 作者:王艳,李雯雯,陈政晓 本文字数:5128字

                      水产检疫论文优秀范文推荐6篇之第五篇:液相芯片技术在水产品检疫性病毒检测技术中的运用  

                        摘要:液相芯片技术是20世纪90年代中期发展起来的, 集合流式细胞技术、激光技术、数字信号处理技术及传统化学技术为一体的新型生物分子检测技术。具有高通量、高灵敏度、高准确度、高精密度、宽的线性范围及操作简便等众多优势, 可用作蛋白质和核酸等生物分子的高通量检测平台。现对液相芯片的发展、原理及该技术在水产品检疫中的应用前景作一简介。

                        关键词:星云棋牌官网下载液相芯片; 水产品; 检疫;

                        YEXIANGXINPIANJISHUSHI20SHIJI90NIANDAIZHONGQIFAZHANQILAIDEBEIYUWEIHOUJIYINZUSHIDAIDEXINPIANJISHU, SHUYUSHENGWUXINPIANDEYIZHONG。YEXIANGXINPIAN, YECHENGWEIWEIQIUTIXUANFUXINPIAN (suspension array, liquid chip) , SHIJIYUxMAP (flexible Multi-Analyte Profiling) JISHUDEXINXINGSHENGWUXINPIANJISHUPINGTAI[1,2,3], TASHIZAIBUTONGYINGGUANGBIANMADEWEIQIUSHANGJINXINGKANGYUAN-KANGTI、MEI-DIWU、PEITI-SHOUTIDEJIEHEFANYINGJIHESUANZAJIAOFANYING, TONGGUOHONG、LVLIANGSHUJIGUANGFENBIEJIANCEWEIQIUBIANMAHEBAOGAOYINGGUANGLAIDADAODINGXINGHEDINGLIANGDEMUDE, YIGEFANYINGKONGNEIKEYIWANCHENGDUODA100ZHONGBUTONGDESHENGWUXUEFANYING[4], SHIJIJIYINXINPIAN、DANBAIXINPIANZHIHOUDEXINYIDAIGAOTONGLIANGFENZIJIANCEJISHUPINGTAI。QIJINWEIZHI10NIANSHIJIAN, QUANQIUYIYOUSHUBAITAOJIYUxMAPJISHUDEJIANCEPINGTAIYONGYUMIANYIXUE、DANBAIZHI、HESUANJIANCE、JIYINYANJIUDENGLINGYU, GAIJISHUYICHENGWEIYIZHONGXINDEDANBAIZHIZUXUEHEJIYINZUXUEYANJIUGONGJU, YESHIZUIZAOTONGGUOMEIGUOSHIPINYUYAOPINGUANLIJU (FDA) RENZHENGDEKEYONGYULINCHUANGZHENDUANDESHENGWUXINPIANJISHU。

                      水产检疫

                      星云棋牌官网下载   ZAISHUICHANPINJIANYIXINGBINGDUJIANCEJISHUZHONG, WOGUOJIANYANJIANYIXITONGDUIYUSHUICHANPINBINGDUSUOCAIYONGDEJIANCEJISHUSHOUDUANZHUYAOBAOKUOPCR、RT-PCRDENGFENZISHENGWUXUEJIANCESHOUDUAN。ZIPCRJISHUFAMINGHOU, YOUYUQIGAOXIAOXINGHE GAO LING MIN DU, DUO ZHONGPCRJIAN CEFANGFAYIBEIYINGYONGDAOSHUICHANPINBINGDUDEJIANCEZHONG, DANSHIZHEIXIEJIANCEJISHUTONGCHANGMEICIZHINENGJIANCEYIZHONGBINGDU, WUFAMANZUKUAISUTONGGUANDEYAOQIU。ZAIDUICHURUJINGDESHUICHANPINJINXINGJIANYIDEGUOCHENGZHONGSUOYINGYONGDEJIANCEJISHUXUYAODADAOZHUNQUE、KUAISU、JIANBIAN、GAOTONGLIANGSIXIANGYAOQIU, QIZHONGZHUNQUEZUIWEIZHONGYAO, YINWEIBUZHUNQUEDEJIANCEKENENGHUIDAOZHILIANGGUODEMAOYIZHENGDUAN;QICISHIKUAISU, ZHEIDUIYUZUDANGZHONGYAOBINGYUANDERUQIN、MANYANDENGWENTIQIDAOLEZHIGUANZHONGYAODEZUOYONG。DANGZHUNQUEHEKUAISUJUNNENGDEDAOMANZUSHI, SHENGSHIJIBIANJIEDECAOZUOBUZHOUZESHILINGYIGEZHONGYAODEYAOQIU。

                        1 液相芯片技术原理[5,6,7]

                        YEXIANGXINPIANTIXIYOUXUDUODAXIAOJUNYIDEYUANXINGWEIQIU (ZHIJING5.5~5.6 μm) WEIZHUYAOJIZHIGOUCHENG, MEIZHONGWEIQIUSHANGGUDINGYOUBUTONGDETANZHENFENZI, JIANGZHEIXIEWEIQIUXUANFUYUYIGEYEXIANGTIXIZHONG, JIUGOUCHENGLEYIGEYEXIANGXINPIANXITONG, LIYONGZHEIGEXITONGKEYIDUITONGYIGEYANGPINZHONGDEDUOZHONGBUTONGFENZITONGSHIJINXINGJIANCE。

                        ZAIYEXIANGXITONGZHONG, WEILEQUFENBUTONGDETANZHEN, MEIYIZHONGGUDINGYOUTANZHENDEWEIQIUDOUYOUYIGEDUTEDESECAIBIANHAO, HUOCHENGYINGGUANGBIANMA。ZAIWEIQIUZHIZAOGUOCHENGZHONGCHANRULEHONGSEHECHENGSE2ZHONGYINGGUANGRANLIAO (ZHEI2ZHONGRANLIAOGEYOU10ZHONGBUTONGQUFEN) , CONGERBAWEIQIUFENWEI100ZHONGBUTONGDEYANSE, XINGCHENGYIGEJUYOUDUTEGUANGPUDIZHIDEHANYOU100ZHONGBUTONGWEIQIUDEZHENLIE。BUTONGYANSEWEIQIUZAIFENLEIJIGUANGJIFAXIACHANSHENGDEYINGGUANGHUBUXIANGTONG, ZHEIZHONGFENLEIYINGGUANGSHISHIBIEBUTONGWEIQIUDEWEIYITUJING。LIYONGZHEI100ZHONGWEIQIU, KEYIFENBIEBIAOJISHANG100ZHONGBUTONGDETANZHENFENZI。

                        JIANCESHIXIANHOUJIARUYANGPINHEBAOGAOFENZIYUBIAOJIWEIQIUFANYING, YANGPINZHONGDEMUDEFENZI (DAIJIANCEDEKANGYUANHUOKANGTI、SHENGWUSUBIAOJIDEBAHESUANPIANDUAN、MEIDENG) NENGGOUYUTANZHENHEBAOGAOFENZITEYIXINGJIEHE, SHIJIAOLIANTANZHENDEWEIQIUXIEDAISHANGBAOGAOFENZIZAOHONGDANBAI, SUIHOULIYONGYIQI (RULuminex100) DUIWEIQIUJINXINGJIANCEHEJIEGUOFENXI。Luminex100CAIYONGWEILIUJISHUSHIWEIQIUKUAISUDANLIETONGGUOJIANCETONGDAO, BINGSHIYONGHONGSEHELVSELIANGZHONGJIGUANGFENBIEDUIDANGEWEIQIUSHANGDEFENLEIYINGGUANGHEBAOGAOFENZISHANGDEBAOGAOYINGGUANGJINXINGJIANCE。HONGSEJIGUANGKEJIANGWEIQIUFENLEI, CONGERJIANDINGGEGEBUTONGDEFANYINGLEIXING (JIDINGXING) ;LVSEJIGUANGKEQUEDINGWEIQIUSHANGJIEHEYINGGUANGFENZIDESHULIANG (JIDINGLIANG) .YINCI, TONGGUOHONGLVSHUANGSEJIGUANGDETONGSHIJIANCE, WANCHENGDUIFANYINGDESHISHI、DINGXINGHEDINGLIANGFENXI。

                      星云棋牌官网下载   JUBENYIXIWEIQIUGENJUBIAOMIANDAIYOUDEFENZICANJIDEBUTONG, KEYIJIEHEBUTONGDETANZHENFENZI, RUDAIYOUSUOJIDEWEIQIUKETONGDANBAIHUOHESUANJIEHE, DAIYOUNi-NTADEWEIQIUKETONGDAIYOUZUANSUANBIAOQIANDEDANBAIJIEHE。KEYIGENJUXUYAOXUANZE。

                        2 液相芯片技术的应用

                        2.1 YEXIANGXINPIANJISHUZAIDANBAIJIANCEZHONGDEYINGYONG

                        BIAOMIANDAIYOUSUOJIDEJUBENYIXIWEIQIU (RUMulti AnalyteWEIQIUHESeroMapWEIQIU) , ZAIEDCHENHSDEHUOHUAHOUKEYITONGDANBAIFENZIDEANJIXINGCHENGGONGJIAJIAN, YOUCIKEYIBAOBEIDANBAITANZHEN, JIANCEYANGBENZHONGDEMUDEFENZI。MUQIANGAIJISHUZHUYAOYINGYONGYUXIBAOYINZIDEJIANCE[8,9,10,11,12,13,14,15], de JagerDENG[16]SHIYONGYEXIANGXINPIANJISHUJIANCELEJINGGUOKANGYUANHESILIEYUANCIJIHOUWAIZHOUXUEDANHEXIBAOSUOFENMIDE15ZHONGXIBAOYINZI, FENXIJIEGUOBIAOMINGYEXIANGXINPIANJISHUPINEIBIANYIXIAOYU10%, PIJIANBIANYIZAI10%~20%FANWEI, BICHUANTONGMEILIANMIANYIFA (enzyme linked immune sorbent assay, ELISA) GENGMINGANHEZHUNQUE。JohannisssonDENG[17]YINGYONGYEXIANGDANBAIXINPIANFANGFAJIANCEZHUCUYANZHENGXIBAOYINZI (proinflammatory cytokines) , LINGMINDUKEDA0.18~12 ng/mL.

                        2.2 YEXIANGXINPIANJISHUZAIHESUANJIANCEZHONGDEYINGYONG

                        DAIYOUSUOJIDEWEIQIUZAIMESHEEDCDEHUOHUAHOUKETONGHESUANTANZHENXIANGLIANJIE, HUOZHESHIYONGDAIYOUanti-tagBIAOQIANDEWEIQIU, JIANGDAIYOUtagDEHESUANTANZHENYUZHIJIEHE, YANGBENTONGWEIQIUSUOXIEDAIDETANZHENZAJIAO, XITUOWEIJIEHEDEDNA, LIANQINHESUBIAOJIDEZAOHONGDANBAIZUOWEIBAOGAOYINGGUANG。FultonDENG[2]SHIYONGDUOTONGDAOJINGZHENGXINGZAJIAOFENXIDUIHLADENGWEIJIYINJINXINGFENXING。TAMENHECHENGLE16GEYUHLA-DQA1DENG2GEWAIXIANZINEIDENGWEIJIYINXULIEDUIYINGDEGUAHEGANSUANZUOWEITANZHEN。YUTANZHENXULIEHUBUDEBIAOJIGUAHEGANSUAN (JINGZHENGXINGGUAHEGANSUAN) XIANYUWEIBIAOJIDESHUANGLIANMOBANYUZAJIAO, ZAIYUFUZAILEYUDENGWEIJIYINHUBUDETANZHENDEWEIQIUXITONGJINXINGZAJIAO, RANHOUSHIYONGLuminex FlowMetrixXITONGJINXINGYINGGUANGFENXI, YUBUJIABIAOJIJINGZHENGWUSHISUOHUODEYINGGUANGQIANGDUJINXINGBIJIAO, QUEDINGCHUYINGGUANGDEYIZHILV。DunbarDENG[18]CAIYONGPCRJISHUKUOZENGCHUDAIJIANBAFENZIDNAHOU, TONGGUOYEXIANGFANYING, XIANGBADNAOULIANTEYIXINGBUHUOXULIE, ZAIYUWEIQIUBIAOMIANDEHUBUXUNZHITANZHENZAJIAOHOUJINXINGJIANCE。CHUCIZHIWAI, YEXIANGXINPIANJISHUHAIYINGYONGYUDANHEGANSUANDUOTAIXINGDEFENXI、ZUZHIXIANGRONGXINGKANGYUANFENXING、SHUIANWURANZHONGZHISHIJUNDEJIANCEJIBINGYUANJUNJIANCEDENGDUOFANGMIANYANJIU。

                        3 液相芯片技术的优缺点

                        YEXIANGXINPIANJISHUZUIDAYOUDIANZAIYUGAOTONGLIANG, JIANCE96KONGYANGBENZHIXU35~60 min, DUIDANYIYANGBENTONGSHIKEJINXINGDUODA100ZHONGDEBUTONGFENXI。ZHENDUIDANYIFENXI, KEYIYICIJIANCE100GESHUJU, MANZUTONGJIXUEYAOQIU。DUIYUELISAERYAN, MEIBIAOBANDEYIGEKONGZHINENGBAOBEIYIZHONGKANGYUAN、ERYEXIANGXINPIANJISHUZHONGMEIZHONGWEIQIUYOUBUTONGDEYINGGUANGRANSE, KEYIBEIJIGUANGTEYIXINGDESHIBIECHULAI, SUOYIKEYIJIANGBAOBEIBUTONGTANZHENFENZIDEWEIQIUHUNHEZAIYIGELVMOBANKONGZHONGTONGSHIJINXINGGEZHONGDANBAIHEHESUANDEFENXI[19].

                        QICIZAIYULINGMINDUGAO、TEYIXINGQIANG、ZHONGFUXINGHAO, LIANGSHUJIGUANGKETONGSHIFENBIEJIANCEWEIQIUFENLEIYINGGUANGHEBAOGAOYINGGUANG, ZHIYOUYUFENLEIYINGGUANGTONGSHICHUXIANDEBAOGAOYINGGUANGXINHAOCAINENGBEIJIANCEJILU, XINZAOBIGAO, TONGSHIZHIXUYAOJISHAOLIANGYANGPINJIKEJINXINGJIANCE。DUIWEIQIUBIAOMIANJINXINGTEYIXINGDEHUAXUECHULI, KEJINYIBUTIGAOTEYIXING。ZAIZHE, YEXIANGXINPIANDEFANYINGSHIZAIYEXIANGZHONGJINXINGDE, YINERFANYINGSHIJIANDUAN、CAOZUOJIANBIAN、KUAIJIE;TONGSHI, YEXIANGDEHUANJINGJIDANBAIZHIDEJIANCETIGONGLEYIGEWENDINGDEHUANJING, GENGYOULIYUWEICHIDANBAIZHIDETIANRANGOUXIANG。

                      星云棋牌官网下载   SHIYANZHENGMING, ZAIDANBAIFENXIFANGMIAN, YEXIANGXINPIANJISHUYUELISAXIANGBIJIAO, JUYOUTONGYANGDETEYIXING, BINGQIEYEXIANGXINPIANDELINGMINDUSHICHANGGUIDEELISAFANGFADELINGMINDU10~100BEI[20].ZAIHESUANFENXIFANGMIAN, YEXIANGXINPIANJISHUYUXULIECEDINGGEYOUTEDIAN, BINIXIANYINJI (reverse line blot, RLB) JUYOUGENGGAODEMINGANXING。

                      星云棋牌官网下载   DANYEXIANGXINPIANJISHUYOUYIGEBIJIAODADEQUEDIAN, JIUSHIDANBAIFENXIGUOCHENGZHONG, YOUYUBUFENXUEQINGZHONGHANYOUSHIYIXINGKANGTI, KEYUWEIQIUHUOBUHUOKANGTIZHIJIELIANJIE, CONGERXINGCHENGFEITEYIXINGDEBEIJINGZHI。DANZHEIZHONGFEITEYIYINGXIANGKEYITONGGUOSHEDINGNEIDUIZHAOHESHIYONGZUDUANYELAIXIAOCHU。

                        4 液相芯片技术在水产品病毒病检疫中的应用展望

                        YEXIANGXINPIANJISHUSHIYIGEHENLINGHUODECAOZUOPINGTAI, YANJIUZHEKEYIGENJUYANJIUXUYAOZENGTIANJIANCEXIANGMU, QIEJIANCEZHUNQUE, XINXIZHILIANGWENDING, KEZHONGFUXINGHAO。YEXIANGXINPIANJISHUZAIJICHUXUEYANJIURUMIANYIFENXIFANGMIANYUNYONGZUIWEIGUANGFAN, CIWAIZAILINCHUANGZHENDUAN, SHIPINWEISHENGJIANDUDENGJUNYOUYINGYONG。JIANYUZAIWOGUOJINCHUKOUSHUICHANPINJIANYIZHONG, JINGCHANGHUIDUITONGYIYANGPINJINXINGDUOZHONGYIBINGDEJIANCE, RUGUONENGJIANGYEXIANGXINPIANYINGYONGDAOSHUICHANPINBINGDUJIANYIZHONG, NEIMEZHIXUHENDUANDESHIJIANJIKESHIXIANTONGSHIDUIDUOGEYANGPINJINXINGDUOZHONGBINGDUDEGAOTONGLIANGJIANCE, YINERYOUWANGDADATIGAOKOUANJIANYIXINGSHUICHANBINGDUDEJIANCEXIAOLVHELINGMINDU。

                        参考文献
                        [1]Bortolin S, Black M, Modi H, et al.Analytical validation of the tagit high-throughout microsphere-based universal array genotyping plat-form:application to the multiplex detection of a panel of thrombo-philia-associated single-nucleotide polymorphisms[J].Clin Chem, 2004, 50 (11) :2028-2036.
                        [2]Fulton R, J McDade R L, Smith P L, et al.Advanced multiplexedanalysis with the FlowMetrix system[J].Clin Chem, 1997, 43 (9) :1749-1756.
                        [3]Yang L, Tran D K, Wang X.BADGE, Beads array for the detectionof gene expression, a high througput diagnostic bioassay[J].Ge-nome Res, 2001, 11:1888-1898.
                        [4]Sun K, Wang Q, Huang X H, et al.Establishment of mulitplexedmicrosphere-based flow cytometric assay for multiple human tumormarkers[J].Acta Pharm Sin, 2007, 28 (12) :2011-2018.
                        [5]Carson R T, Vignali D A A.Simultaneous quantitation of 15 cyto-kines using a multiplexed flow cytometric assay[J].J ImmunolMeth, 1999, 227:41-52.
                        [6]Hadd A G, Laosinchai-Wolf W, Novak C R.Microsphere beadarrays and sequence validation of 5/7/9T genotypes for multiplexscreening of cystic fibrosis polymorphisms[J].J Mol Diagnos, 2004, 6:348-355.
                        [7]Hulse R E, Kunkler P E, Fedynyshyn J P.Optimization of multi-plexed bead-based cytokine immunossays for rat serum and brain tis-sue[J].J Neuroscience Meth, 2004, 136:87-98.
                        [8]Carson R T, Vignali D A A.Simultaneous quantitation of 15 cyto-kines using a multiplexed flow cytometric assay[J].J ImmunolMeth, 1999, 227:41-52.
                        [9]Hadd A G, Laosinchai-Wolf W, Novak C R.Microsphere beadarrays and sequence validation of 5/7/9T genotypes for multiplexscreening of cystic fibrosis polymorphisms[J].J Mol Diagnos, 2004, 6:348-355.
                        [10]Hulse R E, Kunkler P E, Fedynyshyn J P.Optimiztion of multi-plexed bead-based cytokine immunoassays for rat serum and braintissue[J].J Neuroscience Meth, 2004, 136:87-98.
                        [11]Kellar K L, Douglass J P.Multiplexed microsphere-based flow cyto-metric immunoassays for human cytokines[J].J Immunol Meth, 2003, 279:277-285.
                        [12]Martins T B, Pasi B M, Pickering J W.Determination of cytokineresponses using a multiplexed fluorescent microsphere immunoassay[J].J Clin Patho, 2002, 118:346-353.
                        [13]Radstake T R, Roelfs M F, Jenniskens Y M.Expression of toll likereceptors 2 and 4 in rhernatoid synovial tissue and regulation byproinflammatory cytokines interleukin-12 and interleukin 18 via in-terferon-gamma[J].Arthritis&Rheurnation, 2004, 50:3856-3865.
                        [14]Bellisario R, Colinas R J, Pass K A.Simultaneous measurement ofthyroxine (T4) and thyrotropin (TSH) from newborn dried blood-spot specimens using a multiplexed fluorescent microsphere immuno-assay[J].Clin Chem, 2000, 46:1422-1424.
                        [15]Khan S S, Smith M S, Reda D, et al.Multiplex bead array assaysfor detection of soluble cytokines:comparisons of sensitivity andquantitive values among kits from muliplemanufactures[J].Cytom-etry B Clin Cytom, 2004, 61 (1) :35-39.
                        [16]de Jager W, Prakken B J, Bijlsma J W, et al.Improved MultiplexImmunoassay Performance in Human Plasma and Synovial Fluid Fol-lowing Removal of Interfering Heterophilic Antibodies[J].J Immu-nol Methods, 2005, 300 (1-2) :124-135.
                        [17]Johannisson A, Jonasson R, Dernfalk J, et al.Simultaneousdetection of porcine proinflammatory cytokines using multiplex flowcytometry by the xMAP technology.Cytometry A, 2006, 69 (5) :391.
                        [18]Dunbar S A, Jacobson J W.Application of the Luminex Xmap tech-nology for rapid, high-throughout mulitplexed nucleic acid detection[J].Clin Chim Acta, 2006, 363 (1-2) :71-82.
                        [19]姚丽, 张伟, 王姝妹, 等, 液相芯片技术原理及应用简介[J].现代肿瘤医学, 2008, 12 (16) :2196-2198.
                      星云棋牌官网下载   [20]Baigini R E, Sammons D L, Smith J P, et al.Comparison of a mul-tiplexed fluorescent covalent microsphere immunoassay and an en-zyme-linked immunosorbent assay for measurement of human immu-noglobulin G antibodies to anthrax toxins[J].Clin Diagn Lab Im-munol, 2004, 11 (1) :50-55.

                      点击查看>>水产检疫论文(推荐6篇)其他文章
                        王艳,李雯雯,陈政晓,熊炜.液相芯片技术及其在水产病毒病检疫中的应用展望[J].畜牧与兽医,2012,44(10):83-85.
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