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              人流胚胎亲子鉴定如何选择检材案例分析

              时间:2019-01-13 来源:实用妇产科杂志 作者:姬云涛 张振 李晓婷 本文字数:2693字

                DUIYUZAOQIYAOWULIUCHANTAIERYONGYUQINZIJIANDINGDEANLIJINNIANWENXIANBAODAOJIAOSHAO,ERZAISHIJIFAYIWUZHENGJIANDINGGONGZUOZHONG,YAOWULIUCHANPEITAIYONGYUJIANCAIDEANLIQUEZHUNIANZENGDUO。TONGCHANGYIYUANFUCHANKEZAIJINXINGZAOQIYAOWULIUCHANSHIWANGWANGBUKAOLVLIUCHANWUZUOWEIFAYIXUESHENGWUJIANCAISHIYONG,YIBANDUILIUCHANWUBUXUZUOJINYIBUDESHAIXUAN,SHENZHIYOUXIELIUCHANZHEZAIJIAZHONGPAICHULIUCHANWU,ZHEIJIUJIXUYAOYONGZAOQIYAOWULIUCHANPEITAIZUOWEIFAYIXUEQINZIJIANDINGSHENGWUJIANCAIDAILAIBUBIAN,ZENGJIALEHUOQUYOUXIAOJIANCAIDENANDU。BENWENYISHIJIGONGZUOZHONGYUDAODEANJIAN“RENSHEN40TIANRENLIUPEITAIQINZIJIANDING”JINGYANWEILI,DUIZAOQIYAOWURENLIUPEITAIYONGZUOFAYIXUEQINZIJIANDINGSHI,RUHENENGGOUGENGHAODISHOUJIJIANCAI,GENGJIAZHUNQUEDICONGLIUCHANPAICHUZUZHISUIKUAIZHONGZHUNQUEDIXUANQUMUBIAOSHENGWUJIANCAITICHUJIANYI,WEIFUCHANKEYISHENGHEFAYIWUZHENGSIFAGONGZUOZHETIGONGYOUYIDEJIEJIANHECANKAO。

                1、资料与方法

                1.1 资料来源

                2015NIAN5YUEANHUISHIPINGSIFAJIANDINGSUOJIEAN1LIQINZIJIANDING,NVFANG25SUI,YUNQIYUE40TIAN,YU2015NIAN5YUE26RIDAOYIYUANJINXINGYAOWULIUCHAN。

                1.2 样本的处理与DNA提取

              星云棋牌官网下载   DUIHUOQUDEYAOWULIUCHANPEITAIHEZUZHISUIKUAIJINXINGQINGXIGANJING(JIANTU1),XUNZHAOJIANCAIMUBIAO,WEILEQUEDINGJIANCAIDEZHUNQUEXING,FENBIEXUANQULIANGZHONGZUZHISUIKUAI,DI1ZHONGSHIYIMIANGUANGHUALINGYIMIANCUCAODEBUGUIZEXINGZHUANGDEZUZHISUIKUAI,DI2ZHONGSHIZHENGTIRONGMAOZHUANGDEZUZHISUIKUAI(JIANTU2),YONGNIEZIHESHOUSHUDAOPIANFENBIEZAIBUGUIZEXINGZHUANGZUZHISUIKUAIDEGUANGHUAMIAN(JIANTU2JIANTOU1)HECUCAOMIAN(JIANTU2JIANTOU2)、ZHENGTIRONGMAOZHUANGZUZHISUIKUAIBIAOMIAN(JIANTU2JIANTOU3),QIEQUMIANJIYUE0.5 cm2DAXIAODEJIANCAI。WEILEBIMIANQUYANGDEOURANXINGWUCHAGESHE3GEZHONGFUYANGBEN,SUOQUJIANCAIYONGYANKEJIANJIANSUI,FANGRU1.5 mlLIXINGUANZHONG,JIARU200μl 5%Chelex-100(w/v)TIQUYE,FANFUZHENDANGHOU,ZHIYUHENGWENZHENDANGQISHANG,56℃BAOWEN30FENZHONG,ZHONGJIANYAOHUANGHUNYUN2CI,RANHOU100℃BAOWEN5FENZHONG,ZHONGJIANZAIYAOHUANGHUNYUN1CI,ZUIHOU10000 r/mLIXIN3FENZHONG。

              清洗干净的药物流产胚胎组织碎块

              图1 清洗干净的药物流产胚胎组织碎块

              两种不同的药物流产胚胎组织碎块

              图2 两种不同的药物流产胚胎组织碎块

              (箭头1:不规则形状的组织碎块光滑面;箭头2:不规则形状的组织碎块粗糙面;箭头3:整体绒毛状的组织碎块)

                1.3 PCR扩增与电泳检测

                SHANGSHULIXINSHANGQINGYE1μl,CAIYONGZHONGDEMEILIANAGCU-EX22SHIJIHEZAI9700XINGPCRKUOZENGYI(MEIGUOABIGONGSI)SHANGANZHAOSHIJIHECAOZUOSHUOMINGJINXINGFUHEKUOZENG,FANYINGTIXIWEI10μl.SHELIYANGXING(9947A)HEYINXINGDUIZHAO(YINXINGDUIZHAOSHIZHIHANPCRFANYINGTIXIDEKONGBAIGUAN)。KUOZENGJIESHUHOUQU1μlKUOZENGCHANWUYU0.3μlNEIBIAOHE10μlQULIZIJIATAIANHUNHE,3500 rpmLIXIN5FENZHONG,ZHI3130XLXINGYICHUANFENXIYI(MEIGUOABIGONGSI)SHANGQUANZIDONGMAOXIGUANDIANYONG,CollectionRUANJIANSHOUJISHUJU,YONGGene Mapper ID v 3.2RUANJIANJINXINGDENGWEIJIYINFENXINGJIEGUOFENXI。TONGSHIQUPEITAIYIFUHEMUQINXUEYANGZHICHENGDEXUEHENLVZHIPIANJINXINGPCRKUOZENGHEDIANYONGJIANCE。

                2、结果

              星云棋牌官网下载   TONGGUOYUMUQINHEYIFUJIYINWEIDIANBIDUI,KEQUERENFUQINHEMUQINYINGTIGONGDEWEIDIAN,21GECHANGRANSETISTRJIYINZUOHE1GEXINGBIEWEIDIANFENXINGJIEGUOXIANSHI(JIANBIAO1),1ZUYUPEITAISHENGMU21GECHANGRANSETISTRJIYINZUOWANQUANXIANGFU,BINGQIEXINGBIEWEIDIANYESHIYIZHIDE,SHUOMINGDI1ZUQUCAIKENENGJIUSHIMUQINDEYANGBENDNAXINXI;DI2ZUDECHANGRANSETISTRJIYINZUOFENXINGBAOHANLEPEITAISHENGMUQUANBU21GECHANGRANSETISTRJIYINZUODEWEIDIAN,MEIGEJIYINZUODUOCHULAIDEWEIDIANDOUKEYIZAIPEITAIYIFUDECHANGRANSETISTRJIYINZUOFENXINGZHONGZHAODAO,SHUOMINGDI2ZUQUCAISHITAIERHEMUQINDEHUNHEWU,BAOHANMUQINYUTAIERDEHUNHEDNAXINXI;ERDI3ZUDE21GECHANGRANSETISTRJIYINZUOWEIDIANYOUYIBANLAIZISHENGMU,YIBANLAIZIYIFU,SHUOMINGDI3ZUQUCAISHITAIERYANGBEN,HANYOUTAIERZISHENDEDNAXINXI。FENXIBUTONGYAOLIUPEITAIZUZHIBUWEIJIANCAI21GECHANGRANSETISTRJIYINZUOHE1GEXINGBIEWEIDIANJIANCEJIEGUO,TAIERYUYIFUZAISUOYOUWEIDIANDOUFUHEYICHUANGUILV,TAIERYUMUQINZAISUOYOUWEIDIANYEDOUFUHEYICHUANGUILV,TAIERAmelogeninXINGBIEJIYINZUOJIANCHUXHEYRANSETIJIYINWEIDIAN,TISHIWEINANXINGTAIER,JIANBIAO1SUOSHI。

              表1 不同部位检材21个常染色体STR基因座和1个性别位点检测结果

              不同部位检材21个常染色体STR基因座和1个性别位点检测结果

              1组代表不规则形状的组织碎块光滑面;2组代表不规则形状的组织碎块粗糙面;3组代表整体绒毛状的组织碎块

                3、讨论

              星云棋牌官网下载   TAIPANSHIYOUTAIERDECONGMIRONGMAOMOHEMUTIDEJITUIMOGONGTONGZUCHENGDEYUANPANXINGJIEGOU,YOUZILIAORENWEIZAOYUNQIZAITAIPANDETAIERMIANFUGAIYOUZIYANGCENGJIPEIWAIZHONGPEICENGFAYUBINGTUIHUAERCHENGDEPINGHUARONGMAOMO(TAIPANGUANGHUAMIAN)JIYANGMO,WEITAIERCHENGFEN,ERTAIPANDECUCAOMIANSHIYOUBOLIHOUDETAIERCONGMIRONGMAOMOJIMUTIDEJITUIMOZUCHENG。DANZAISHIJIDEZAOQIYAOWULIUCHANZHONG,HUICHUXIANGEZHONGGEYANGDELIUCHANWU,DUIYUDANYIDEYIKUAIMINGXIANDETAINANGZUZHIDEQINGKUANGZEJIAOHAOPANDUANJIANDINGYONGQUCAIBUWEI,RUGUOLIUCHUWUWEIDUOKUAIBUGUIZEPEITAIZUZHISUIKUAI(BENANZHONGQINGKUANG),ZEBIXUTIAOXUANKEYIZHUNQUEYONGYUJIANDINGDEJIANCAI。BENANZHONGDUOGEBUGUIZEPEITAIZUZHISUIKUAIJIBENSHANGDOUSHIYIMIANGUANGHUA、YIMIANCUCAO,QIZHONGZHAODAOLEYIKUAIZHENGTIRONGMAOZHUANGDESUIKUAI,JIANCEJIEGUOBIAOMING,CIZAOQIYAOWULIUCHANWUZHONG,ZHEIZHONGZHENGTIRONGMAOZHUANGDESUIKUAIKEYIZHUNQUETIGONGLIUCHANTAIERDEDNAXINXI,ERQITASUIKUAIWULUNGUANGHUAMIANHAISHICUCAOMIANDOUBUNENGZHUNQUETIGONGLIUCHANTAIERDEDNAXINXI,CUCAOMIANDUOWEITAIERHEMUQINDEHUNHEDNAXINXI,GUANGHUAMIANGENGKENENGTIGONGTAIERMUQINDEDNAXINXI,ZHEIYUYIYOUYANJIUJIEGUOCUNZAIBUTONG,YUANYINKENENGSHISUOHUOLIUCHANPAICHUWUHEQUCAIBUTONGSUOZHI。GAIYANJIUBAODAOSUOHUOLIUCHANWUWEIYI6 cm×7 cmDAXIAOTAIPAN,CITAIPANYIMIANGUANGHUA(PINGHUARONGMAOMOMIAN),YIMIANCUCAO(TUIMOMIAN),CONGGUANGHUAMIANJICUCAOMIANGEQUCAILIANGCHU,YICICONGBIAOMIANGUAQULVDOULIDAXIAOZUZHIHE0.2 cm×0.2 cm×0.5 cmSHENBUZUZHIKUAI,QIJIANCEJIEGUOBIAOMING,CUCAOMIANGUAQUWUDEDNAFENXINGYUMUQINYIZHI,ERGUANGHUAMIANGUAQUWUDEDNAFENXINGSHITAIERDE,RENWEISHIYONGTAIPANJINXINGQINZIJIANDING,QISHOUXUANBUWEIYINGSHITAIPANGUANGHUAMIANDEGUAQUWU,QUTAIPANJIAOSHENBUWEIDEDAODEYANGPINWEIMUQINHETAIERHUNHEPIN。HAIYOUWENXIANBAODAOLERENSHEN40TIANTAIERRONGMAOZUZHIQINZIJIANDING1LI,DANGAIWENXIANMEIYOUSHUOMINGRUHEPANDUANSHAIXUANTAIERRONGMAOZUZHI。

                ZAOQIYAOWURENLIUDOUZAIHUAIYUN7ZHOUYIQIANJINXING,JIUSHIYINWEITAIERYUTAIPANDOUHAICHUYUBUWENDINGDEZHUANGTAI。ZHEISHIBAOGUOZAISHOUJINGLUANWAICEDERONGMAOHAIMEIYOUYUZIGONGNEIMOXINGCHENGWENDINGLIANJIE,LIUCHANSHITONGCHANGHUICHENGYOULIZHUANGTAICUNZAI。YIBANYAOWULIUCHANPEITAIZUZHIPAICHUSHI,YOUYUTAIPANTAIERDOUMEIYOUCHENGXING,ZHISHIJIANDANDEXUEKUAIYANGRONGMAOZUZHI,TONGSHIBANYOUBUTONGSHULIANGHEDAXIAODEZIGONGTUIMOSUIKUAI,TAINANGHUNZAIQIZHONGWANGWANGBUYITIAOXUAN。TAINANGSHIGANGGANGFAYUDEPEITAIZUZHI,JINGGUOYAOWUCHULILIUCHANCHULAISHISHIXUEKUAIYANGJIEGOU,XUEKUAIZHUANGDETAINANGZAIQINGSHUIZHONGPIAOXIBIANHUICHENGWEIRONGMAOZHUANG,KEYIKANDAOBAISEDERONGMAO,DANSHIBUYIFENBIANCHULAI。TAINANGWANZHENGDEHUA,SHIBAISERONGMAOQIUYANGDE,HUOCHENGZHANGTIAOZHUANG,ZHIJINGDAXIAOYUEZAI1~2 cmFANWEI。DANGTAIERBUFENDERONGMAOZUZHIBUMINGXIANSHI,JIUJIZHEIYIFAYIXUESHENGWUJIANCAIDEHUOQUDAILAINANDU。

              星云棋牌官网下载   MUQIAN,YOUGUANZAOQITAIPANZUZHIRUHEQUCAIYONGYUQINZIJIANDINGDEBAODAOFEICHANGYOUXIAN,TEBIESHIZAOQIYAOWULIUCHANWUZUOWEISHENGWUXUEJIANCAIDEWENXIANBAODAOHENSHAO。ERBIZHESHIJIGONGZUOZHONGYUDAODUOQIXUYAOTONGGUOZAOQIYAOWURENLIUPEITAIQINZIJIANDINGDEANJIAN,YOUYUYIYUANFUCHANKEYISHENGYUFAYIWUZHENGQINZIJIANDINGDANWEIZAIYEWUSHANGMEIYOUJIAOJI,BUNENGCONGZAOQIYAOWULIUCHANWUZHONGZHUNQUESHAIXUANCHUTAINANG,CHANGCHANGSHIDECONGYIYUANNADAODEZAOQIYAOWULIUCHANWUBUNENGHENHAODISHIYONGYUFAYIWUZHENGQINZIJIANDINGSHENGWUXUEJIANCAI。ZHEIJIUYAOQIUCONGSHIJIANDINGDERENYUANYEXUYAOJUYOUZUGOUDEJINGYAN,NENGGOUZHUNQUEDICONGZAOQIYAOWULIUCHANWUZHONGTIAOXUANCHUMUBIAOJIANCAIYONGYUFAYIWUZHENGQINZIJIANDING。BENANDEJIEGUOBIAOMING,ZAIZHONGDUODELIUCHANPEITAIZUZHISUIKUAIZHONGXUNZHAORONGMAOTUANYANGJIEGOU,KENENGKEYIGENGZHUNQUEDIXUANQUMUBIAOSHENGWUJIANCAI,HUOQUTAIERDEDNAXINXI,WEIGUANGDAFUCHANKEYISHENGHEFAYIWUZHENGJIANDINGRENYUANTIGONGJIEJIANHECANKAO。

                姬云涛,张振,李晓婷,李士平,屈长青.早期药物流产胚胎组织用于亲子鉴定1例[J].实用妇产科杂志,2016,32(05):396-397.
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